Calcium hydroxide associated with a new vehicle: Psidium cattleianum leaf extracts. Tissue response evaluation.

The aim of this study was to evaluate edemogenic activity and subcutaneous inflammatory reaction induced by Psidium cattleianum leaf extracts associated with Ca(OH)2. Thirty male Wistar rats, split equally into three groups [aqueous extract + Ca(OH)2; ethanolic extract + Ca(OH)2; and propylene glycol + Ca(OH)2], were assessed every 3 h or 6 h (five animals in each period). Under general anesthesia, 0.2 mL of 1% Evans blue per 100 g of body weight was injected into the penile vein and each combination to be evaluated was subcutaneously injected into the dorsal region 30 min thereafter. Edemogenic activity was analyzed by spectrophotometry (λ=630 nm). For inflammatory reaction analysis, 50 rats received four polyethylene tubes (three experimental groups) and an empty tube (control group). The assessments were made at 7, 15, 30, 60, and 90 days, followed by hematoxylin-eosin staining and by the assignment of scores for evaluation of tissue response intensity. Ethanolic extract + Ca(OH)2 yielded the largest edemogenic activity at 3 h. Intergroup differences at 6 h were not significant. The histological analysis showed progressive repair over time (p<0.05) and aqueous and ethanolic extracts produced similar responses to those of the control and Ca(OH)2 + propylene glycol groups. Psidium cattleianum leaf extracts used as Ca(OH)2 vehicles evoked similar tissue response when compared to Ca(OH)2 associated with propylene glycol.

Mixing failures of endodontic sealers: an in vivo biocompatibility study / Falhas durante espatulação de cimentos endodônticos: um estudo de biocompatibilidade in vivo

Objectives: Evaluate, in vivo, the influence of mixing failures on endodontic sealers. Material and Methods: To alveolus analysis, 80 rats were divided into Sealapex® and AH Plus® groups. Within each group, the sealer was subjected to either partial (incomplete homogenization­ simulating handling failures) or total mixing (complete homogenization) over two periods of 7 and 30 days (n = 20). The maxillary incisor was extracted and a polyethylene tube containing the sealer was inserted. To quantify edema, 40 male rats were divided into four groups (n = 10). The animals received 2% Evans Blue intravenously, and either AH Plus® or Sealapex® was injected subcutaneously. The rats were euthanized after 3 or 6 hours and analyzed in a spectrophotometer (630 ƞm). To analyze the subcutaneous tissue, 20 rats received polyethylene tube implants with the sealers in the dorsal area (n=10), then euthanized after either 7 or 30 days, and inflammation was evaluated according to an inflammatory cells score. Results: In the alveolar 7-day group, control group presented an inflammation score 1, while all other groups presented a score 2, except AH plus® total mix group (3). After 30 days, all groups presented a score 1. The edemogenic test showed less edema in Sealapex® groups (p < 0.5). In subcutaneous 7-day period, all groups presented score 2. In 30 days, all groups revealed score 1, except AH Plus® partial mix group (2). Conclusion: Regarding mixing of the sealers, there were no significant differences among the groups (AU)

Objetivo: Avaliar, in vivo, a influência das falhas de espatulação de cimentos endodônticos. Material e Métodos: Para análise alveolar, 80 ratos foram divididos nos grupos Sealapex® e AH Plus®. Em cada grupo, o cimento foi espatulado de forma parcial (homogeneização incompleta, simulando falhas) ou total (homogeneização completa) em dois períodos de 7 e 30 dias (n=20). O incisivo superior foi extraído e um tubo de polietileno contendo o cimento foi inserido. Para quantificar edema, 40 ratos foram divididos em quatro grupos (n = 10). Os animais receberam Azul de Evans 2% intravenoso, e AH Plus® ou Sealapex® injetados no tecido subcutâneo. Após 3 ou 6 horas foram eutanasiados e analisados em espectrofotômetro (630 ƞm). Para analisar a resposta subcutânea, 20 ratos receberam implantes de tubo de polietileno com os cimentos na região dorsal (n = 10), eutanasiados após 7 ou 30 dias, e a inflamação foi avaliada de acordo com um escore de células inflamatórias. Resultados: Na análise alveolar em 7 dias, o grupo controle apresentou escore 1 de inflamação, enquanto que todos os outros grupos apresentaram 2, com exceção do AH plus® espatulação total (3). Após 30 dias, todos os grupos apresentaram escore 1. O teste edemogênico mostrou menor edema nos grupos Sealapex® (p < 0,5). No período subcutâneo de 7 dias, todos os grupos apresentaram escore 2. Em 30 dias, todos os grupos revelaram escore 1, exceto AH Plus® espatulação parcial (2). Conclusão: Não houve diferença estatística significante entre os cimentos quanto à espatulação. (AU)

Calcium hydroxide associated with a new vehicle: Psidium cattleianum leaf extracts. Tissue response evaluation

Abstract The aim of this study was to evaluate edemogenic activity and subcutaneous inflammatory reaction induced by Psidium cattleianum leaf extracts associated with Ca(OH)2. Thirty male Wistar rats, split equally into three groups [aqueous extract + Ca(OH)2; ethanolic extract + Ca(OH)2; and propylene glycol + Ca(OH)2], were assessed every 3 h or 6 h (five animals in each period). Under general anesthesia, 0.2 mL of 1% Evans blue per 100 g of body weight was injected into the penile vein and each combination to be evaluated was subcutaneously injected into the dorsal region 30 min thereafter. Edemogenic activity was analyzed by spectrophotometry (λ=630 nm). For inflammatory reaction analysis, 50 rats received four polyethylene tubes (three experimental groups) and an empty tube (control group). The assessments were made at 7, 15, 30, 60, and 90 days, followed by hematoxylin-eosin staining and by the assignment of scores for evaluation of tissue response intensity. Ethanolic extract + Ca(OH)2 yielded the largest edemogenic activity at 3 h. Intergroup differences at 6 h were not significant. The histological analysis showed progressive repair over time (p<0.05) and aqueous and ethanolic extracts produced similar responses to those of the control and Ca(OH)2 + propylene glycol groups. Psidium cattleianum leaf extracts used as Ca(OH)2 vehicles evoked similar tissue response when compared to Ca(OH)2 associated with propylene glycol.

Avaliação da resposta tecidual e da capacidade de mineralização de cimentos endodônticos resinosos / Biocompatibility and biomineralization assessment of resinous root canal sealers

Objetivo: Avaliar a resposta tecidual e a capacidade de biomineralização dos materiais endodônticos SK Seal Root Canal Sealer (SK Seal), Sealer 26® e AH plus® em tecido subcutâneo de ratos. Material e Métodos: Vinte e quatro ratos Wistar (n=6) receberam implantes subcutâneo contendo os cimentos e um tubo vazio como controle. Após 7, 15, 30 e 60 dias, os animais foram eutanasiados e os tubos de polietileno foram removidos junto com o tecido circunjacente. Em seguida, os espécimes foram processados para análise em Hematoxilina-Eosina, von Kossa, luz polarizada e imunoistoquímica para fibronectina (FN) e tenascina (TN). Os dados foram tabulados e analisados através do teste de Kruskal-Wallis e Dunn (p<0,05). Resultados: Todos os materiais testados induziram uma reação inflamatória moderada aos 7 e 15 dias (p> 0,05). Não foram observadas diferenças entre os grupos após 30 ou 60 dias (p> 0,05). A cápsula fibrosa foi considerada espessa aos 7 dias, tornando-se fina no final do experimento. Todos os grupos apresentaram marcadores positivos para FN e TN em todos os tempos de análise, com maior imunomarcação para os cimentos em comparação ao grupo controle (p <0,05). Os cimentos não apresentaram von Kossa positiva ou estruturas birrefringentes à luz polarizada. Conclusão: Todos os cimentos testados apresentaram biocompatibilidade, porém não estimularam a mineralização(AU)

Aim: The aim of this study was to evaluate biocompatibility and biomineralization of the endodontic materials SK Seal Root Canal Sealer (SK Seal), Sealer 26® and AH plus® in subcutaneous tissue of rats. Methodology: Twenty-four Wistar rats (n=6) received subcutaneous implants containing the test sealers, and an empty tube as control. After 7, 15, 30 and 60 days, the animals were killed and polyethylene tubes were removed with the surrounding tissues. The pieces were processed for Hematoxylin-Eosin, von Kossa, polarized light and immunohistochemical analysis for fibronectin (FN) and tenascin (TN). Data were tabulated and analyzed via Kruskal-Wallis and Dunn's test (p<0,05). Results: All tested materials induced a moderate infammatory reaction after 7 and 15 days. (p>0,05). No difference was observed among groups after days 30 or 60 days (p>0.05). The fibrous capsule was considered thick on the 7th day, and classified as thin at the end of the experiment. All groups presented positive markers for FN and TN in all analyzed time, with higher immunolabeling to sealers in comparison with the control group (p<0,05). The sealers did not present von Kossa positive or birefringent structures to polarized light. Conclusion: All tested sealers demonstrated biocompatibility, but did not stimulate the mineralization(AU)

Biocompatibility and biomineralization assessment of bioceramic-, epoxy-, and calcium hydroxide-based sealers.

Obturation of the root canal system aims to fill empty spaces, promoting hermetic sealing and preventing bacterial activity in periapical tissues. This should provide optimal conditions for repair, stimulating the process of biomineralization. An endodontic sealer should be biocompatible once it is in direct contact with periapical tissues. The aim of this study was to evaluate the rat subcutaneous tissue response to implanted polyethylene tubes filled with Smartpaste Bio, Acroseal, and Sealapex and investigate mineralization ability of these endodontic sealers. Forty Wistar rats were assigned to the three sealers groups and control group, (n = 10 animals/group) and received subcutaneous implants containing the test sealers, and the control group were implanted with empty tubes. After days 7, 15, 30, and 60, animals were euthanized and polyethylene tubes were removed with the surrounding tissues. Inflammatory infiltrate and thickness of the fibrous capsule were histologically evaluated. Mineralization was analyzed by Von Kossa staining and polarized light. Data were tabulated and analyzed via Kruskal-Wallis and Dunn's test. All tested materials induced a moderate inflammatory reaction in the initial periods. Smartpaste Bio induced the mildest inflammatory reactions after day 15. No difference was observed among groups after days 30 or 60. Von Kossa-positive staining and birefringent structures observed under polarized light revealed a larger mineralization area in Sealapex-treated animals followed by Smartpaste Bio-treated animals. At the end of the experiment, all tested sealers were found to be biocompatible. All sealers induced biomineralization, except Acroseal, which induced a mild tissue reaction.

Biocompatibility and biomineralization assessment of bioceramic-, epoxy-, and calcium hydroxide-based sealers

Abstract Obturation of the root canal system aims to fill empty spaces, promoting hermetic sealing and preventing bacterial activity in periapical tissues. This should provide optimal conditions for repair, stimulating the process of biomineralization. An endodontic sealer should be biocompatible once it is in direct contact with periapical tissues. The aim of this study was to evaluate the rat subcutaneous tissue response to implanted polyethylene tubes filled with Smartpaste Bio, Acroseal, and Sealapex and investigate mineralization ability of these endodontic sealers. Forty Wistar rats were assigned to the three sealers groups and control group, (n = 10 animals/group) and received subcutaneous implants containing the test sealers, and the control group were implanted with empty tubes. After days 7, 15, 30, and 60, animals were euthanized and polyethylene tubes were removed with the surrounding tissues. Inflammatory infiltrate and thickness of the fibrous capsule were histologically evaluated. Mineralization was analyzed by Von Kossa staining and polarized light. Data were tabulated and analyzed via Kruskal-Wallis and Dunn's test. All tested materials induced a moderate inflammatory reaction in the initial periods. Smartpaste Bio induced the mildest inflammatory reactions after day 15. No difference was observed among groups after days 30 or 60. Von Kossa-positive staining and birefringent structures observed under polarized light revealed a larger mineralization area in Sealapex-treated animals followed by Smartpaste Bio-treated animals. At the end of the experiment, all tested sealers were found to be biocompatible. All sealers induced biomineralization, except Acroseal, which induced a mild tissue reaction.

Contribuição da radiografia panorâmica no diagnóstico da osteoporose / Contribution of the panoramic radiograph on the diagnosis of the osteoporosis

Considerada como a epidemia silenciosa do século pela Organização Mundial de Saúde, a osteoporose afeta milhões de pessoas em todo o mundo. A perda de osso ocorre progressivamente e muitas vezes sem sintomas, até que uma fratura ocorra pela primeira vez. A densitometria é hoje o exame de referência para o diagnóstico da osteoporose. A osteoporose também pode apresentar manifestações na cavidade oral e, baseando-se nesses achados, alguns estudos têm procurado demonstrar que a radiografia panorâmica pode auxiliar no diagnóstico da osteoporose. Este trabalho tem como objetivo discutir a utilização da radiografia panorâmica no diagnóstico precoce da osteoporose por meio de revisão de literatura

Considered the silent epidemic of the century by the World Health Organization, the osteoporosis affects millions of people worldwide. The bone loss occurs gradually and often without symptoms until a fracture occurs. Densitometry is today the reference test for the diagnosis of osteoporosis. Osteoporosis can also present manifestations in the oral cavity and, based on these findings, some studies have attempted to demonstrate that the panoramic radiograph can assist in the diagnosis of osteoporosis. This study aims to discuss the use of panoramic radiography in early diagnosis of osteoporosis by means of literature review